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Isolates in the Phytophthora megasperma
complex have been assigned to different
species of Phytophthora. To resolve this
controversy in Iran, the morphology, physiology
and isozyme electerophoretic patterns of 40 isolates
attributable to P. megasperma
complex obtained mainlyfrom pistachiotrees, soybeans,alfalfa,
sainfoinand pine trees
were compared. Electerophoretic patterns of nine isozymes
including: esterase,
diaphorase, glucose-6-phosphatedehydrogenase,glucosephosphate
isomerase,isocitrate
dehydrogenase, lactate dehydorgenase, malate
dehydrogenase, malic enzyme and
superoxide dismutase were analized using vertical
discontinuous acrylamide gel.
On the basis of sporangium and oogonium morphology and size,
P. megasperma
isolates from various hosts were not accuratelydelineatedits to
taxonomicgroups. Based
on growth patterns on various media
(CV-8,MEA,CNiA,SAMA),growthrate at 20°C
and cardinal temperatures for growth,P. megasperma.isolatescould
also be separated to
taxonomic groups.